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KMID : 1025520050470040615
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Journal of Animal Science and Technology
2005 Volume.47 No. 4 p.615 ~ p.624
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Study on Rumen Cellulolytic Bacterial Attachment and Fermentation Dependent on Initial pH by cPCR
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Kim M.-S.
Sung H.-G.
Kim H.-J.
Lee S.-S.
Chang J.-S.
Ha Jong-Kyu
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Abstract
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The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.
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KEYWORD
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Initial medium pH, F. succinogenes, Attachment, Rumen fermentation, cPCR
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